SEMSTER-VI
Long Essay 10 marks
| 1.Explain the different methods of immobilization of enzyme. And give its applications |
| 2.Describe the production of hepatitis B vaccine. |
| 3.Explain the production of Griseofulvin and Penicillin by fermentation technology with a neat labelled flow chart. |
| 4.What are biosensors? Explain the types with pharmaceutical applications. |
| 5.Write a detailed note on cloning vectors. |
| 6.Describe the production of citric acid by fermentation technology with a neat labelled flow chart. |
| 7.Explain different methods of enzyme immobilisation with their advantages and disadvantages. |
| 8.Describe the production of Hepatitis B vaccine. Write a note on plasmid vectors. |
| 9.Describe the production of Penicillin by fermentation technology with a neat labelled flow chart. |
| 10.What are biosensors? Write its principle and its functions. |
| 11.Write in detail the production of Insulin by genetic engineering method. |
| 12.Write in detail the different types of fermenters and its applications. |
| 13.Write in detail the different methods of enzyme immobilization. Write its applications. |
| 14.Write in detail three different vectors used in genetic engineering. |
| 15.Write the design of an industrial fermenter with a neat labelled diagram. |
| 16.What is enzyme immobilization? Explain covalent bonding and gel entrapment methods with advantages and disadvantages. |
| 17.Describe the general method of recombinant DNA technology. |
| 18.Describe the construction and working of a fermenter with a neat labelled diagram. |
| 19.Describe any three methods of enzyme immobilization. Illustrate the applications of immobilised enzymes in medicine and production of pharmaceuticals. |
| 20.Describe the production of insulin by recombinant DNA technology. |
| 21.Describe the different methods of fermentation. Explain the production of griseofulvin by fermentation technology with a neat labelled flow chart. |
| 22.What are biosensors? Explain the types with pharmaceutical applications. |
| 23.Write a detailed note on cloning vectors. |
| 24.Describe the production of citric acid by fermentation technology with a neat labelled flow chart. |
| 25.Explain different methods of enzyme immobilisation with their advantages and disadvantages. |
| 26.Describe the production of Hepatitis B vaccine. Write a note on plasmid vectors. |
| 27.Describe the production of Penicillin by fermentation technology with a neat labelled flow chart. |
| 28.Explain different types of biosensors and their applications in pharmacy and medicine. |
| 29.Describe the production of insulin by r DNA technology. |
| 30.Describe the production of Penicillin by fermentation technology with a neat labelled flow chart. |
Short Essays 5 marks
| 1.Describe the production and uses of Penicillinase. |
| 2.Explain the production of interferons by rDNA technology. |
| 3.Describe the production of hormone insulin. |
| 4.Describe the structure and functions of MHC. |
| 5.Describe the production of monoclonal antibodies by hybridoma technology. |
| 6.Explain the general method of the preparation of viral vaccine. |
| 7.Write a note on serum –immune blood derivatives. |
| 8.Describe ELISA test with its applications. |
| 9.What is Mutation and explain the different types of mutation. |
| 10.Describe the production and uses of amylase. |
| 11.Explain the production of interferons by rDNA technology. |
| 12.List out the applications of genetic engineering in medicine. |
| 13.Write a note on microbial biotransformation in production of steroidal medicinal agents. |
| 14.List out different blood products and their applications. |
| 15.Outline the general method for the production of live viral vaccines. |
| 16.Write the role of lymphocytes in immunity. |
| 17.Describe the Southern blot test. How does it differ from Western blot test? |
| 18.Explain conjugation. Write its significance. |
| 19.Describe the production and uses of lipase. |
| 20.Explain polymerase chain reaction with applications. |
| 21.Outline the production of interferons by rDNA technology |
| 22.What is biotransformation? List out the applications of microbial biotransformation. |
| 23.Describe the applications and method for production of monoclonal antibodies. |
| 24.Outline the general method for the preparation of live attenuated bacterial vaccines. |
| 25.What are vaccines? Classify the types with examples. |
| 26.Describe the Southern blot test with its applications. |
| 27.Explain transduction and conjugation. |
| 28.How to make sodium alginate beads in calcium chloride solution? |
| 29.What is PCR? Write the working principle of PCR. |
| 30.Explain the structure of immunoglobulins. |
| 31.Write the production ofkilled bacterial vaccines. |
| 32.Write the production of monoclonal antibodies. |
| 33.Write in brief the western blotting technique and mention its application. |
| 34.Write in brief about transformation with suitable examples. |
| 35.Write briefly about different types of mutation and give its significance. |
| 36.Write in brief the Collection, Processing and Storage of whole human blood. |
| 37.Explain the method for determination of immobilized amylaseactivity. |
| 38.Write a note on applications of genetic engineering in medicine production. |
| 39.Write a note on Immune stimulation and Immune suppression. |
| 40.Write in brief structure and function of MHC. |
| 41.Write a note on storage and stability of vaccines. |
| 42.Write in brief the southern blot technique and mention its applications. |
| 43.Write in brief about transduction. Explain the types. |
| 44.Describe ELISA with its applications. |
| 45.Define fermentation and write the production of Vitamin B12. |
| 46.Describe the production and uses of amylase. |
| 47.Explain the production of insulin by rDNA technology. |
| 48.Describe the technique of polymerase chain reaction (PCR). |
| 49.Explain the production of penicillin G by fermentation technology. |
| 50.Classify immunity. Write the difference between active and passive immunity |
| 51.Outline the general method for the preparation of live attenuated bacterial vaccines. |
| 52.Describe the structure of an immunoglobulin with a neat labelled diagram. |
| 53.Describe ELISA with its applications. |
| 54.Explain microbial biotransformation with examples. |
| 55.Describe the production and uses of penicillinase. List out advantages of 56.production of enzymes from microbial sources. |
| 57.Explain the production of hepatitis B vaccine by rDNA technology. |
| 58.What are restriction enzymes? Explain the types and their role in genetic engineering. |
| 59.Explain different types of mutations. |
| 60.Describe the production of monoclonal antibodies by hybridoma technology. |
| 61.Outline the general method for the preparation of bacterialtoxoids. |
| 62.What are vaccines? Classify the types with examples. |
| 63.Describe the Southern blot test with its applications. |
| 64.What is transduction. Explain the methods. |
| 65.Describe the production and uses of amylase. |
| 66.Explain the production of interferons by rDNA technology. |
| 67.List out the applications of genetic engineering in medicine. |
| 68.Write a note on microbial biotransformation in production of steroidal medicinal agents. |
| 69.List out different blood products and their applications. |
| 70.Outline the general method for the production of live viral vaccines. |
| 71.Write the role of lymphocytes in immunity. |
| 72.Describe the Southern blot test. How does it differ from Western blot test? |
| 73.Explain conjugation. Write its significance. |
| 74.Describe the production and uses of lipase. |
| 75.Explain Polymerase chain reaction with applications. |
| 76.Outline the production of interferons by rDNA technology. |
| 77.What is biotransformation? List out the applications of microbial biotransformation. |
| 78.Describe the applications and method for production of monoclonal antibodies. |
| 79.Outline the general method for the preparation of antitoxin. |
| 80.Describe the structure of Immunoglobulin. |
| 81.Describe ELISA with its applications. |
| 82.Explain different types of mutations. |
| 83.Describe the production and uses of catalase. |
| 84.What are cloning vectors? Explain the features of pBR322. |
| 85.What are molecular scissors. Explain their role in recombinant DNA technology. |
| 86.Explain the production of antibodies from B lymphocytes. |
| 87.Classify vaccines giving examples.Write the difference between toxins and toxoids. |
| 88.What are monoclonal antibodies? Describe different methods of purification of MAb. |
| 89.Describe ELISA with its applications. |
| 90.Explain briefly, transformation, transduction and conjugation. |
| 91.Explain different types of mutagenic agents. |
Short Answers 2 marks
| 1. How is fermentation equipments sterilized. |
| 2.Write any two application of Microbial Biotransformation. |
| 3.Define conjugation. |
| 4.Define plasmids with examples. |
| 5.Define immunoglobulins. |
| 6.Write the storage conditions of vaccines. |
| 7.Difference between vaccine and serum. |
| 8.What is PCR. Give two applications. |
| 9.Write any four applications of Amylase. |
| 10.List out any four advantages of Enzyme Immobilization. |
| 11.List out any two applications of enzymes in medicine. |
| 12.Write any four methods of immobilisation techniques. |
| 13.Define immunity. List the types of immunity. |
| 14.What are different types of aerators. |
| 15.List out different types of hypersensitivity reactions. |
| 16.Write any four properties of Immunoglobulin M. |
| 17.Write the difference between vaccines and sera |
| 18.What are cosmid vectors? |
| 19.Write any four applications of PCR. |
| 20.Write any four differences between prokaryotic and eukaryotic DNA. |
| 21.List out any four applications of enzymes in medicine. |
| 22.Write any four applications of immobilised enzymes. |
| 23.What is cellular immunity? |
| 24.What is protected fermentation? |
| 25.What is immune suppression? Give two example for immunosuppressive agent. |
| 26.Write any four properties of Immunoglobulin G. |
| 27.What are the conditions for storage of official vaccines? |
| 28.What are transposons? |
| 29.Write any two applications of ELISA. |
| 30.What do you mean by plasmid. |
| 31.Name any four natural polymers used for immobilization. |
| 32.Name any four disadvantages of immobilization. |
| 33.Write any four applications of interferons. |
| 34.Define toxoids.Give two examples. |
| 35.Name any twoblood products with applications. |
| 36.What is hypersensitivity? Write the types. |
| 37.What is microbial biotransformation? Give two examples. |
| 38.What is downstream processing? Give two examples. |
| 39.Expand ELISA and write two applications. |
| 40.Applications of plasma substitutes. |
| 41.Name any four advantages of enzyme immobilization. |
| 42.Name any four chemical polymers used for immobilization. |
| 43.What are restriction endonuclease enzymes? Give two examples. |
| 44.Name two methods of preparation of viral vaccine. |
| 45.Define hybridoma technology. Write two applications | |
| 46.Write the therapeutic uses of plasma substitutes | . |
| 47.Name four mutagenic agent. | |
| 48.What is upstream processing? Give two examples. | |
| 49.What are transposons and episomes? | |
| 50.Write any four pharmaceutical applications of biosensors. | |
| 51.Write any four pharmaceutical applications of biotechnology. | |
| 52.Enlist the different types of vectors used in genetic engineering. | |
| 53.Write the source organism and uses of griseofulvin. | |
| 54.What are anti-toxins? Give an example. | |
| 55.Write any two functions of MHC. | |
| 56.What are plasma substitutes? | |
| 57.Write any four applications of western blotting? | |
| 58.What are point mutation? | |
| 59.List out any four applications of enzymes in medicine. | |
| 60.Write any four advantages of immobilised enzymes. | |
| 61.What is cellular immunity? | |
| 62.What is protected fermentation? | |
| 63.What is immune suppression? Give two examples for immunosuppressive agent. | |
| 64.Write any four properties of Immunoglobulin G. | |
| 65.What are the conditions for storage of official vaccines? | |
| 66.What are transposons? | |
| 67.Write any two applications of ELISA. | |
| 68.List out any two applications of enzymes in medicine. | |
| 69.Mention anytwo methods of immobilisation techniques. | |
| 70.Define immunity. List the types of immunity | |
| 71.What are different types of aerators. | |
| 72.List out different types of hypersensitivity reactions. | |
| 73.Write any four properties of Immunoglobulin M. | |
| 74.What is HAT medium. Write its role in selection of hybridoma cells. | |
| 75.What are cosmid vectors? | |
| 76.Write any two applications of PCR. | |
| 77.List out any four applications of biotechnology in medicine. | |
| 78.Write any four synthetic polymers used forimmobilisation of enzymes | |
| 79.What is humoral immunity? | |
| 80.List any four factors that affect fermentation process. | |
| 81.Write the functions of MHC. | |
| 82.Write any four properties of Immunoglobulin A. | |
| 83.Write the difference between vaccines and sera. | |
| 84.Write the difference between transposons and episomes. | |
| 85.Write any four applications of Southern blot test. | |
86.What is protein engineering? | |
| 87.Write any four applications of proteases | |
| 88.What is chimeric DNA? | |
| 89.What are batch culture and continuous cultures? | |
| 90.Write any four uses of blood products. | |
| 91.Write the role of CD4 cell in immune reaction. | |
| 92.Write the different types of heavy chains and light chains in immunoglobulin molecules | |
| 93.Write the importance of transposons. | |
| 94.Write any two enzymes and their substrates employed in ELISA. | |
| 95.What are plasmids. Give one example. | |