SEMSTER-VI
Long Essay 10 marks
1.Explain the different methods of immobilization of enzyme. And give its applications |
2.Describe the production of hepatitis B vaccine. |
3.Explain the production of Griseofulvin and Penicillin by fermentation technology with a neat labelled flow chart. |
4.What are biosensors? Explain the types with pharmaceutical applications. |
5.Write a detailed note on cloning vectors. |
6.Describe the production of citric acid by fermentation technology with a neat labelled flow chart. |
7.Explain different methods of enzyme immobilisation with their advantages and disadvantages. |
8.Describe the production of Hepatitis B vaccine. Write a note on plasmid vectors. |
9.Describe the production of Penicillin by fermentation technology with a neat labelled flow chart. |
10.What are biosensors? Write its principle and its functions. |
11.Write in detail the production of Insulin by genetic engineering method. |
12.Write in detail the different types of fermenters and its applications. |
13.Write in detail the different methods of enzyme immobilization. Write its applications. |
14.Write in detail three different vectors used in genetic engineering. |
15.Write the design of an industrial fermenter with a neat labelled diagram. |
16.What is enzyme immobilization? Explain covalent bonding and gel entrapment methods with advantages and disadvantages. |
17.Describe the general method of recombinant DNA technology. |
18.Describe the construction and working of a fermenter with a neat labelled diagram. |
19.Describe any three methods of enzyme immobilization. Illustrate the applications of immobilised enzymes in medicine and production of pharmaceuticals. |
20.Describe the production of insulin by recombinant DNA technology. |
21.Describe the different methods of fermentation. Explain the production of griseofulvin by fermentation technology with a neat labelled flow chart. |
22.What are biosensors? Explain the types with pharmaceutical applications. |
23.Write a detailed note on cloning vectors. |
24.Describe the production of citric acid by fermentation technology with a neat labelled flow chart. |
25.Explain different methods of enzyme immobilisation with their advantages and disadvantages. |
26.Describe the production of Hepatitis B vaccine. Write a note on plasmid vectors. |
27.Describe the production of Penicillin by fermentation technology with a neat labelled flow chart. |
28.Explain different types of biosensors and their applications in pharmacy and medicine. |
29.Describe the production of insulin by r DNA technology. |
30.Describe the production of Penicillin by fermentation technology with a neat labelled flow chart. |
Short Essays 5 marks
1.Describe the production and uses of Penicillinase. |
2.Explain the production of interferons by rDNA technology. |
3.Describe the production of hormone insulin. |
4.Describe the structure and functions of MHC. |
5.Describe the production of monoclonal antibodies by hybridoma technology. |
6.Explain the general method of the preparation of viral vaccine. |
7.Write a note on serum –immune blood derivatives. |
8.Describe ELISA test with its applications. |
9.What is Mutation and explain the different types of mutation. |
10.Describe the production and uses of amylase. |
11.Explain the production of interferons by rDNA technology. |
12.List out the applications of genetic engineering in medicine. |
13.Write a note on microbial biotransformation in production of steroidal medicinal agents. |
14.List out different blood products and their applications. |
15.Outline the general method for the production of live viral vaccines. |
16.Write the role of lymphocytes in immunity. |
17.Describe the Southern blot test. How does it differ from Western blot test? |
18.Explain conjugation. Write its significance. |
19.Describe the production and uses of lipase. |
20.Explain polymerase chain reaction with applications. |
21.Outline the production of interferons by rDNA technology |
22.What is biotransformation? List out the applications of microbial biotransformation. |
23.Describe the applications and method for production of monoclonal antibodies. |
24.Outline the general method for the preparation of live attenuated bacterial vaccines. |
25.What are vaccines? Classify the types with examples. |
26.Describe the Southern blot test with its applications. |
27.Explain transduction and conjugation. |
28.How to make sodium alginate beads in calcium chloride solution? |
29.What is PCR? Write the working principle of PCR. |
30.Explain the structure of immunoglobulins. |
31.Write the production ofkilled bacterial vaccines. |
32.Write the production of monoclonal antibodies. |
33.Write in brief the western blotting technique and mention its application. |
34.Write in brief about transformation with suitable examples. |
35.Write briefly about different types of mutation and give its significance. |
36.Write in brief the Collection, Processing and Storage of whole human blood. |
37.Explain the method for determination of immobilized amylaseactivity. |
38.Write a note on applications of genetic engineering in medicine production. |
39.Write a note on Immune stimulation and Immune suppression. |
40.Write in brief structure and function of MHC. |
41.Write a note on storage and stability of vaccines. |
42.Write in brief the southern blot technique and mention its applications. |
43.Write in brief about transduction. Explain the types. |
44.Describe ELISA with its applications. |
45.Define fermentation and write the production of Vitamin B12. |
46.Describe the production and uses of amylase. |
47.Explain the production of insulin by rDNA technology. |
48.Describe the technique of polymerase chain reaction (PCR). |
49.Explain the production of penicillin G by fermentation technology. |
50.Classify immunity. Write the difference between active and passive immunity |
51.Outline the general method for the preparation of live attenuated bacterial vaccines. |
52.Describe the structure of an immunoglobulin with a neat labelled diagram. |
53.Describe ELISA with its applications. |
54.Explain microbial biotransformation with examples. |
55.Describe the production and uses of penicillinase. List out advantages of 56.production of enzymes from microbial sources. |
57.Explain the production of hepatitis B vaccine by rDNA technology. |
58.What are restriction enzymes? Explain the types and their role in genetic engineering. |
59.Explain different types of mutations. |
60.Describe the production of monoclonal antibodies by hybridoma technology. |
61.Outline the general method for the preparation of bacterialtoxoids. |
62.What are vaccines? Classify the types with examples. |
63.Describe the Southern blot test with its applications. |
64.What is transduction. Explain the methods. |
65.Describe the production and uses of amylase. |
66.Explain the production of interferons by rDNA technology. |
67.List out the applications of genetic engineering in medicine. |
68.Write a note on microbial biotransformation in production of steroidal medicinal agents. |
69.List out different blood products and their applications. |
70.Outline the general method for the production of live viral vaccines. |
71.Write the role of lymphocytes in immunity. |
72.Describe the Southern blot test. How does it differ from Western blot test? |
73.Explain conjugation. Write its significance. |
74.Describe the production and uses of lipase. |
75.Explain Polymerase chain reaction with applications. |
76.Outline the production of interferons by rDNA technology. |
77.What is biotransformation? List out the applications of microbial biotransformation. |
78.Describe the applications and method for production of monoclonal antibodies. |
79.Outline the general method for the preparation of antitoxin. |
80.Describe the structure of Immunoglobulin. |
81.Describe ELISA with its applications. |
82.Explain different types of mutations. |
83.Describe the production and uses of catalase. |
84.What are cloning vectors? Explain the features of pBR322. |
85.What are molecular scissors. Explain their role in recombinant DNA technology. |
86.Explain the production of antibodies from B lymphocytes. |
87.Classify vaccines giving examples.Write the difference between toxins and toxoids. |
88.What are monoclonal antibodies? Describe different methods of purification of MAb. |
89.Describe ELISA with its applications. |
90.Explain briefly, transformation, transduction and conjugation. |
91.Explain different types of mutagenic agents. |
Short Answers 2 marks
1. How is fermentation equipments sterilized. |
2.Write any two application of Microbial Biotransformation. |
3.Define conjugation. |
4.Define plasmids with examples. |
5.Define immunoglobulins. |
6.Write the storage conditions of vaccines. |
7.Difference between vaccine and serum. |
8.What is PCR. Give two applications. |
9.Write any four applications of Amylase. |
10.List out any four advantages of Enzyme Immobilization. |
11.List out any two applications of enzymes in medicine. |
12.Write any four methods of immobilisation techniques. |
13.Define immunity. List the types of immunity. |
14.What are different types of aerators. |
15.List out different types of hypersensitivity reactions. |
16.Write any four properties of Immunoglobulin M. |
17.Write the difference between vaccines and sera |
18.What are cosmid vectors? |
19.Write any four applications of PCR. |
20.Write any four differences between prokaryotic and eukaryotic DNA. |
21.List out any four applications of enzymes in medicine. |
22.Write any four applications of immobilised enzymes. |
23.What is cellular immunity? |
24.What is protected fermentation? |
25.What is immune suppression? Give two example for immunosuppressive agent. |
26.Write any four properties of Immunoglobulin G. |
27.What are the conditions for storage of official vaccines? |
28.What are transposons? |
29.Write any two applications of ELISA. |
30.What do you mean by plasmid. |
31.Name any four natural polymers used for immobilization. |
32.Name any four disadvantages of immobilization. |
33.Write any four applications of interferons. |
34.Define toxoids.Give two examples. |
35.Name any twoblood products with applications. |
36.What is hypersensitivity? Write the types. |
37.What is microbial biotransformation? Give two examples. |
38.What is downstream processing? Give two examples. |
39.Expand ELISA and write two applications. |
40.Applications of plasma substitutes. |
41.Name any four advantages of enzyme immobilization. |
42.Name any four chemical polymers used for immobilization. |
43.What are restriction endonuclease enzymes? Give two examples. |
44.Name two methods of preparation of viral vaccine. |
45.Define hybridoma technology. Write two applications | |
46.Write the therapeutic uses of plasma substitutes | . |
47.Name four mutagenic agent. | |
48.What is upstream processing? Give two examples. | |
49.What are transposons and episomes? | |
50.Write any four pharmaceutical applications of biosensors. | |
51.Write any four pharmaceutical applications of biotechnology. | |
52.Enlist the different types of vectors used in genetic engineering. | |
53.Write the source organism and uses of griseofulvin. | |
54.What are anti-toxins? Give an example. | |
55.Write any two functions of MHC. | |
56.What are plasma substitutes? | |
57.Write any four applications of western blotting? | |
58.What are point mutation? | |
59.List out any four applications of enzymes in medicine. | |
60.Write any four advantages of immobilised enzymes. | |
61.What is cellular immunity? | |
62.What is protected fermentation? | |
63.What is immune suppression? Give two examples for immunosuppressive agent. | |
64.Write any four properties of Immunoglobulin G. | |
65.What are the conditions for storage of official vaccines? | |
66.What are transposons? | |
67.Write any two applications of ELISA. | |
68.List out any two applications of enzymes in medicine. | |
69.Mention anytwo methods of immobilisation techniques. | |
70.Define immunity. List the types of immunity | |
71.What are different types of aerators. | |
72.List out different types of hypersensitivity reactions. | |
73.Write any four properties of Immunoglobulin M. | |
74.What is HAT medium. Write its role in selection of hybridoma cells. | |
75.What are cosmid vectors? | |
76.Write any two applications of PCR. | |
77.List out any four applications of biotechnology in medicine. | |
78.Write any four synthetic polymers used forimmobilisation of enzymes | |
79.What is humoral immunity? | |
80.List any four factors that affect fermentation process. | |
81.Write the functions of MHC. | |
82.Write any four properties of Immunoglobulin A. | |
83.Write the difference between vaccines and sera. | |
84.Write the difference between transposons and episomes. | |
85.Write any four applications of Southern blot test. | |
86.What is protein engineering? | |
87.Write any four applications of proteases | |
88.What is chimeric DNA? | |
89.What are batch culture and continuous cultures? | |
90.Write any four uses of blood products. | |
91.Write the role of CD4 cell in immune reaction. | |
92.Write the different types of heavy chains and light chains in immunoglobulin molecules | |
93.Write the importance of transposons. | |
94.Write any two enzymes and their substrates employed in ELISA. | |
95.What are plasmids. Give one example. | |